1. The DNA fragments separated on an agarose gel can be visualised after staining with:

A. Acetocarmine
B. Aniline blue
C. Ethidium bromide
D. Bromophenol blue
Answer :   Ethidium bromide
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2. In bacterial chromosomes, the nucleic acid polymers are

A. linear DNA molecule
B. circular DNA molecule
C. of two types-DNA and RNA
D. linear RNA molecule
Answer :   circular DNA molecule
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3. DNA or RNA segment tagged with a radioactive molecule is called

A. Vector
B. Probe
C. Clone
D. Plasmid
Answer :   Probe
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4. What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis?

A. The smaller the fragment size, the farther it moves
B. Positively charged fragments move to farther end
C. Negatively charged fragments do not move
D. The larger the fragment size, the farther it moves
Answer :   The smaller the fragment size, the farther it moves
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5. The correct order of steps in Polymerase Chain Reaction (PCR) is

A. Extension, Denaturation, Annealing
B. Annealing, Extension, Denaturation
C. Denaturation, Annealing, Extension
D. Denaturation, Extension, Annealing
Answer :   Denaturation, Annealing, Extension
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6. Introduction of food plants developed by genetic engineering is not desirable because

A. economy of developing countries may suffer
B. these products are less tasty as compared to the already existing products
C. this method is costly
D. there is danger of entry of viruses and toxins with introduced crop
Answer :   there is danger of entry of viruses and toxins with introduced crop
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7. What is antisense technology?

A. A cell displaying a foreign antigen used for synthesis of antigens
B. Production of somaclonal variants in tissue cultures
C. When a piece of RNA that is complementary in sequence is used to stop expression of a specific gene
D. RNA polymerase producing DNA
Answer :   When a piece of RNA that is complementary in sequence is used to stop expression of a specific gene
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8. Which of the following is commonly used as a vector for introducing a DNA fragment in human lymphocytes?

A. Retrovirus
B. Ti plasmid
C. pBR 322
D. $$\lambda $$ phage
Answer :   Retrovirus
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9. The plasmid

A. helps in respiration
B. genes found inside nucleus
C. is a component of cell wall of bacteria
D. is the genetic part in addition to DNA in micro-organisms
Answer :   is the genetic part in addition to DNA in micro-organisms
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10. Bt cotton variety that was developed by the introduction of toxin gene of Bacillus thuringiensis (Bt) is resistant to

A. Fungal diseases
B. Plant nematodes
C. Insect predators
D. Insect pests
Answer :   Insect pests
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